Reactions of normal and leukemic cell surfaces to a wheat germ agglutinin.
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چکیده
منابع مشابه
Reactions of normal and leukemic cell surfaces to a wheat germ agglutinin.
A variety of differences between normal and leukemic cells have been reported, involving such diverse characteristics as alkaline phosphatase content,"' 12 glycolytic rate, and nucleic acid metabolism.6 In most cases, however, the differences observed could have been due to variations in cell maturation levels rather than to leukemia-specific changes. We still do not know whether leukemia resul...
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seems clear that the input virus alone provided the stimulus." The close association of foci of giant cells with sites of subsequent transformation may also be related to this chronic infectious process; if the giant cells are sites of maximal virus growth, the neighboring cells may be most affected. In this connection, Shein et al.3 noted that nuclei of the early giant cells consistently showe...
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Embryonic tissue cells dissociated with ethylenediaminetetraacetate are readily agglutinated by the carbohydrate-binding protein concanavalin A. In this property, they resemble transformed, neoplastic cells; and they differ from untransformed adult cells, which are agglutinated by concanavalin A only after their receptors are unmasked by proteolytic treatment. Receptor sites for wheat germ aggl...
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Procedures for the isolation, purification, and crystallization of wheat germ agglutinin are described. The agglutinin was purified 184-fold to homogeneity from commercial wheat germ lipase. A molecular weight of 23,500 was estimated for the protein by means of sedimentation equilibrium and sodium dodecyl sulfate gel electrophoresis. The agghxtinin is a glycoprotein. Amino acid and carbohydrate...
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The effects of wheat germ agglutinin on Drosophila embryonic cell lines growing on cover-glasses was examined by scanning electron microscopy. At low concentrations of the lectin (5-10 mug/ml), cells spread against the glass surface and fused to form syncytia. At high concentration, damage to the cell surface was evidenced as extensive membrane shrivelling and loss of surface microfilaments. Fu...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1965
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.54.2.400